Transcriptome analysis reveals loss of HSPC quiescence in +37kb^HOM mice. (A) Volcano plot showing genes differentially expressed in HSPCs of +37kb^HOM (n = 3) compared with +37kb^WT mice (n = 3). Differentially expressed genes are represented as log2 fold change (x-axis) and log10 P value (y-axis). Significantly upregulated genes (log2 fold change >1.5, P value < .05) are shown in red, downregulated genes (log2 fold change <−1.5, P value < .05) are shown in blue, and genes without significant differences are shown in gray. (B) Mean and SD of Cebpa expression in +37kb^WT and +37kb^HOM LSKs, expressed as FPKM values. (C) Bar plot showing the top downregulated neutrophil-associated genes in +37kb^HOM HSPCs compared with +37kb^WT HSPCs, presented as log2 fold change. (D) GSEA enrichment plot (left) showing upregulation (NES = 2.22; FDR <0.05) of the early myeloid-biased gene expression program in +37kb^HOM HSPCs. Heatmap (right) showing significant differentially expressed genes of this pathway in +37kb^HOM vs +37kb^WT HSPCs. (E) Bar plot showing downregulation of LT-HSC quiescence-related pathways in +37kb^HOM vs +37kb^WT HSPCs, expressed as -log2 transformation of FDR. (F) GSEA enrichment plot (left) showing downregulation (NES = −2.52; FDR <0.05) of the HSC quiescence pathway from³  in +37kb^HOM vs +37kb^WT HSPCs. Heatmap (right) of the significant differentially expressed genes in this dataset. (G) GSEA enrichment plot (left) showing downregulation (NES = −2.22; FDR <0.05) of the ribosome pathway and heatmap (right) of differentially expressed ribosomal Rsp and Rlp genes. (H) Mean and SD of Mycn expression in +37kb^WT and +37kb^HOM HSPCs, expressed as FPKM values. The experiment was done in triplicates for each condition, +37kb^WT (n = 3) and +37kb^HOM (n = 3), and the heatmap values were calculated using Z-scores. SD, standard deviation.