Systemic BM perturbations impair myeloid differentiation and deplete the LT-HSC pool of recipient mice. (A) Total BM cellularity per femur, corrected for body weight in grams of each mouse. The mice transplanted with homozygous BM cells were divided into 2 subgroups based on disease severity: homozygous mild and homozygous severe. Mice transplanted with WT BM were used as controls. (B) Histological examination using H&E on processed paraffin BM sections of recipient CD45.1 transplanted mice. (C) Absolute numbers of Mac1+Gr1+ neutrophils from BM of CD45.1 recipient mice. (D) Immunohistochemistry of S100A8 protein expression on histological BM sections of recipient CD45.1 transplanted mice. (E) HSPC absolute numbers in BMs of CD45.1 recipient mice, calculated from LSK/lineage-negative/live cells (7AAD) and corrected for total cellularity per femur and body weight (in grams) of each mouse. (F) LT-HSC absolute numbers in BM of CD45.1 recipient mice, calculated from CD48-Cd150+/LSK/lineage-negative/live cells (7AAD) and corrected for total cellularity per femur and body weight in grams of each mouse. All data are represented as mean +/− SD. Statistical significance was calculated using a Student t-test. *P value < .05; **P value < .005; ***P value < .0005; N.S., not significant; SD, standard deviation.