Figure 2.
GATA2-IRF8 axis differentially regulates fetal liver progenitor populations. (A) Quantitation of progenitor populations in E14.5 fetal livers obtained from 7 litters (−77+/+Irf8+/+, n = 7; −77−/−Irf8+/+, n = 5; −77+/+Irf8−/−, n = 3; −77−/−Irf8+/−, n = 8; −77−/−Irf8−/−, n = 7). (B) mRNA quantitation from sorted Ly6C− GMPs from 4 to 9 embryos obtained from 10 litters. Real-time PCR primers for quantitation of Irf8 transcripts selectively amplified the wild-type, but not mutant, allele. ND, not detected. Error bars for all plots represent mean ± SEM. *P < .05, **P < .01, ***P < .001, ****P < .0001; Welch’s unequal variance t tests.

GATA2-IRF8 axis differentially regulates fetal liver progenitor populations. (A) Quantitation of progenitor populations in E14.5 fetal livers obtained from 7 litters (−77+/+Irf8+/+, n = 7; −77−/−Irf8+/+, n = 5; −77+/+Irf8−/−, n = 3; −77−/−Irf8+/−, n = 8; −77−/−Irf8−/−, n = 7). (B) mRNA quantitation from sorted Ly6C− GMPs from 4 to 9 embryos obtained from 10 litters. Real-time PCR primers for quantitation of Irf8 transcripts selectively amplified the wild-type, but not mutant, allele. ND, not detected. Error bars for all plots represent mean ± SEM. *P < .05, **P < .01, ***P < .001, ****P < .0001; Welch’s unequal variance t tests.

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