Enhanced dendritic cell differentiation of −77^−/− CMPs requires IRF8. (A) Representative flow plots of dendritic cells differentiated from flow-sorted CMPs cultured with GM-CSF, IL-4, and TNFα. (B) Quantitation of dendritic cell populations using early (CD11c) and late (CD86) DC maturation markers. Data were obtained from 3 to 8 embryos (7 litters). The populations were pregated to exclude cell doublets and dead (4′,6-diamidino-2-phenylindole⁺) cells. Error bars represent mean ± SEM. *P < .05, **P < .01, ****P < .0001; Welch’s unequal variance t tests.