siFga attenuates acute phase response following LPS challenge. Mice were treated with siLuc (red) or siFga (blue) 1 week prior to intraperitoneal injection with LPS and euthanized 24 hours later. (A-C) Relative liver Fga (A), Fgb (B), and Fgg (C) mRNA levels in mouse livers, normalized to a housekeeping gene (B2m). (D-E) Plasma fibrinogen (D) and D-dimer (E) levels were quantified by ELISA. (F) TNF-α, IL-1β, IL-17, MCP-1, and MIP-1α levels in plasma were quantified using a multiplex cytokine analysis. n = 9 for panels A, B, and D; n = 6 for panels C and F, and n = 3 for panel E. *P < .05, **P < .01, ***P < .001. ns, no significant difference. Error bars represent mean ± SEM.