siFga restores macrophage migration in plasminogen-deficient mice. Plasminogen-deficient (Plg^−/−) mice were injected with siLuc (blue) or siFga (green) 1 week prior to intraperitoneal injection of thioglycollate and euthanized 72 hours later. Plasminogen WT littermates (Plg^+/+; red) were included as control and were also injected with thioglycollate. (A) Plasma fibrinogen levels were quantified by ELISA. (B-F) Peritoneal lavage fluids were analyzed by flow cytometry for total cell counts (B), monocyte/macrophages (C), T cells (D), B cells (E), and neutrophils (F). n = 5 to 6 all panels. *P < .05, **P < .01, ***P < .001. ns, no significant difference. Error bars represent mean ± SEM.