Fga^270/270 and siFga-treated mice achieve hemostasis following vascular injury. (A) Time to cessation of bleeding (sustained >30 seconds) of Fga^WT/WT, Fga^WT/270, Fga^270/270, and Fga^−/− mice as well as siLuc- and siFga-treated mice following 3-mm excision of the distal portion of the tail. Horizontal bars indicate mean times for each group. Note that 1 of 6 Fga^270/270 mice and 6 of 6 Fga^−/− mice did not stop bleeding during the 5-minute evaluation period. Open dots represent mice in which rebleeding episodes occurred and the values indicate total bleeding time. Kaplan-Meier analysis was used to determine statistical significance. (B) Time to cessation of bleeding of Fga^WT/WT, Fga^WT/270, Fga^270/270, and Fga^−/− mice following 3 consecutive laser-induced saphenous injuries (n = 14-16 per genotype). Two-way ANOVA was used to determine statistical significance. (C) Representative 3-dimensional reconstruction of injury sites depicting the intravascular view 5 minutes following each injury. Each grid box = 50 μm × 50 μm. Quantification of (D) platelet and (E) fibrin accumulation at the site of injury over the course of 3 injuries. **P < .01, ***P < .001, ****P < .0001. ns, no statistical significance.