Fga^270/270 platelets contain higher levels of fibrinogen relative to fibrinogen levels in plasma. (A-D) Platelet lysates and (E-H) plasma harvested from Fga^WT/WT, Fga^WT/270, Fga^270/270, and Fga^−/− mice using antibodies against fibrinogen Bβ-chain (Fib Bβ), fibronectin (Fn), and VWF. Ponceau S staining of albumin (PS) was used as a loading control (n = 3 per genotype). (I-L) Platelet lysates and (M-P) plasma harvested from untreated or siLuc- or siFga-treated mice using antibodies against fibrinogen Bβ-chain, fibronectin, and VWF. Ponceau S staining of albumin was used as a loading control (n = 4-5 per treatment). One-way ANOVA test was used to determine statistical significance. *P < .05, **P < .01, ****P < .0001. ns, no statistical significance.