Altered clotting with Fga^270/270 plasma and FibAα²⁷⁰ protein. (A) TT, (B) PT, and (C) APTT of Fga^WT/WT, Fga^WT/270, 10% Fga^WT/WT, Fga^270/270, and Fga^−/− mice (n = 4-6 per genotype). Kaplan-Meier analysis was used to determine statistical significance. (D) Turbidity analysis of Fga^WT/WT, Fga^WT/270, 10% Fga^WT/WT, and Fga^270/270 plasma (n = 3-6 per genotype). (E) Representative thrombin generation curves and (F-I) associated parameters, and (J) representative plasmin generation curves and (K-N) associated parameters (n = 3-4 per genotype). One-way ANOVA was used to determine statistical significance. Turbidity analysis using (O) 2 mg/mL of purified FibAα^WT and FibAα²⁷⁰ fibrinogen in buffered system and 1 mg/mL of FibAα^WT and FibAα²⁷⁰ fibrinogen reconstituted in Fga^−/− plasma in the (P) absence or (Q) presence of tPA (n = 1 per group). *P < .05, **P < .01, ***P < .001, ****P < .0001. ns, no statistical significance.