Fga^270/270 and siFga-treated mice are protected from venous thrombosis. (A) Thrombus weights from Fga^WT/WT, Fga^WT/270, Fga^270/270, and Fga^−/− mice 24 hours after IVC ligation (stasis model). Kruskal-Wallis test was used to determine statistical significance. (B) Representative images of thrombi isolated 24 hours after IVC ligation. (C) Circulating fibrinogen levels of siLuc- and siFga-treated mice immediately before the IVC ligation, measured by ELISA. Red dots indicate mice that later developed occlusive thrombi. (D) Thrombus weights from siLuc- and siFga-treated mice 24 hours after ligation. Mantel-Cox test was used to determine statistical significance. Time course of (E) FXIII activation and (F) fibrin crosslinking in Fga^WT/WT and Fga^270/270 plasma. HMWP, high-molecular-weight polymer; MWM, molecular weight marker. **P < .01. ns, no statistical significance.