Fga^270/270 and siFga-treated mice have preserved fibrinogen-dependent antimicrobial function. (A) Kaplan-Meier analysis of survival of Fga^WT/WT, Fga^270/270, and Fga^−/− mice after intraperitoneal infection with 0.7 × 10⁹ CFUs of S aureus (n = 8 per genotype). (B) Representative photomicrographs of cytospin preparations of peritoneal lavage fluid 1 hour after intraperitoneal infection with 0.7 × 10⁹ CFUs of S aureus from Fga^WT/WT, Fga^270/270, and Fga^−/− mice (n = 6 per genotype). Note the cell-associated and free bacteria in samples from Fga^−/− mice that are absent in samples from Fga^WT/WT and Fga^270/270 mice. Scale bar: 10 μm. CFUs within (C) lavage fluid, (D) blood, (E) lung homogenates, and (F) heart homogenates after intraperitoneal infection of S aureus in Fga^WT/WT, Fga^270/270, and Fga^−/− mice. CFUs within (G) lavage fluid, (H) blood, (I) lung homogenates, and (J) heart homogenates after intraperitoneal infection with S aureus in untreated, siLuc- and siFga-treated mice. The dashed lines in panels C and G indicate the initial inoculum dose in CFUs. One-way ANOVA test was used to determine statistical significance. *P < .05, **P < .01, ****P < .0001. ns, no statistical significance.