Confirmation of the effectiveness of CAMK2G inhibition in vivo using retroviral BM transplantation model. (A) Sublethally irradiated BALB/c mice were transplanted with c-kit⁺ BM cells transduced with retroviruses carrying the vector of MPL W515L. These mice were treated with intraperitoneal injections of berbamine (BBM) from day 14 to 23. (B) The survival curves for bone marrow transplantation (BMT) model mice treated with berbamine. Log-rank test: *P < .05, N = 4 mice per group. (C) Parameters, including white blood cell count (WBC count), hemoglobin (Hb), and platelet count (PLT), are shown. N = 5 mice per group. (D) Spleen length, spleen weight, and liver weight are shown N = 3 mice per group. ANOVA test: *P < .05. (E-F) The result of the apoptosis assay in peripheral blood cells of MPL W515L-induced MF model mice under BBM treatment. Peripheral blood cells were measured by FACS after Annexin V + propidium iodide (PI) staining on day 24 with or without BBM treatment. Results are means ± SD. N = 3 mice per group. Unpaired 2-tailed Student t test: *P < .05.