Mitochondria and mitochondrial function in MSPCs (p4) from 5-FU-treated O5A^Δ/Δ and control mice. (A-B) Graphs showing 1 experiment out of 2 as an example for extracellular acidification rate (ECAR) (A) and OXPHOS levels measured by oxygen consumption rates (OCR) (B), in cultured MSPCs, respiration maximum (right panel) is shown for MSPCs from both experiments; oligo, oligomycin; FCCP, p-trifluoromethoxyphenylhydrazone; R&A, rotenone and antimycin. (C) Fluorescent microscopy images and intensity of reactive oxygen species (ROS = DCFDA, green) levels and DAPI (blue) in MSPCs. Graph showing pixel intensity measured by ImageJ software. (D) Fluorescent microscopy images and diameter of mitochondria designated as feret's diameter measured by ImageJ software (MitoTrackerRed, red) and DAPI (blue) in MSPCs. (E) Fluorescent microscopy images of mitochondria (TOMM20) in MSPCs illustrated as relief image (Adobe Photoshop: v21.1.1/filter relief) and diameter of mitochondria designated as feret's diameter measured by ImageJ software. Scale bars, 10 µm. *P < .05 (nonparametric Mann-Whitney test; A-E). The results of each panel represent 2 or 3 independent experiments. Data are represented as mean ± SEM. Open symbols and bars represent measurements of control (5A^fl/fl and O5A^+/+). Closed symbols and bars represent measurements of O5A^Δ/Δ MSPCs. Symbol legend shown in Figure 4A.