Heightened and sustained inflammation in CGD. Zymosan was delivered i.p. to WT and gp91^phox-/y mice to induce peritonitis. (A) Mice were monitored for weight loss over time as a percentage of starting weight. (B) Enumeration of neutrophils, Resident peritoneal macrophages, and recruited monocyte-derived macrophages (MoMacs) from peritoneal lavage over time. Cell counts were derived from flow cytometry (as described in supplemental Figure 2) and hemocytometer counting. (C) Ly6C and MHCII expression by WT (top) and CGD (bottom) MoMacs during peritonitis. (D) MoMac cell size over time measured by forward scatter area (FSC-A) and by cytospin. Blue denotes WT cells, red denotes CGD. Filled histograms or dashed lines represent major and minor MoMac populations, respectively. Cytospins were performed at 72 hours post–zymosan injection (post Zym), and images were acquired by using an Olympus BX15 microscope at 40× original magnification (cropped images shown). Graphs show mean per group ± standard error and are representative of 5 experiments. *P < .05, **P < .01.