WT and CGD MoMacs are differently programmed. Genes involved in the following biological processes were identified from RNA-sequencing data, and genes differentially expressed at least twofold between the major population of WT and CGD MoMacs at 72 hours are presented. Heat maps show differential expression of pro-inflammatory genes (A), genes associated with efferocytosis and phagocytosis (B), cell adhesion molecules and integrins (C), and metabolic genes either associated with glycolysis (top) or oxidative metabolism (below) (D). All heat maps are minimum (Min)–maximum (Max) scaled by row and show triplicate samples from the major population of WT (blue) or CGD (red) MoMacs at the indicated time points after zymosan injection. Genes are ranked by differential expression (log₂FC [WT/CGD]) at 72 hours post–zymosan injection (post Zym) so that genes with the greatest differences between genotypes are at the top (higher in WT) and bottom (higher in CGD). Genes marked with an asterisk were chosen for subsequent validation. (E-G) Expression of the indicated proteins by WT and CGD MoMacs over time was confirmed by flow cytometry. Graphs show mean fluorescence intensity (MFI) of antibody staining of the major MoMac population at the time points indicated relative to isotype control staining (±standard error) and representative of 2 independent experiments with total n > 5 mice per group. *P < .05, **P < .01, ***P < .001 comparing WT and CGD at the indicated time point. (H) GLUT1 (Slc2a1) protein expression was confirmed by flow cytometry. Expression of Idh1 transcript was confirmed by quantitative reverse transcription polymerase chain reaction from independently sorted MoMacs pooled from 3 animals; data were normalized to both expression of the 18S ribosomal gene and expression of the target gene by 24-hour WT MoMacs. N.D., not detected.