CGD MoMacs are continuously recruited into the peritoneal cavity. (A) PKH injection was performed at 17 hours post–zymosan injection (post Zym), and PKH-positive (+) and PKH-negative (–) MoMacs were enumerated at the indicated time points from WT (left) and CGD mice (right). (B) Phenotype of PKH⁺ MoMacs over time in WT (top) and CGD mice (bottom). (C) PKH injection was performed at either 48 (left) or 72 (right) hours post Zym, and percent PKH⁺ MoMacs were analyzed 1 or 6 hours later; data shown are normalized to the percent labeling 1 hour after PKH injection. All data shown are mean ± standard error of the mean and represent n = 5 to 8 mice per group, summarized from at least 2 independent experiments. ***P < .001 comparing WT and CGD.