CGD MoMac turnover is not the result of cell death or migration to lymph nodes, circulation, or omenta. (A) Percentages of apoptotic (right) and necrotic (left) MoMacs were calculated from peritoneal lavage at the indicated times post–zymosan injection (post Zym) by staining with Annexin V and Sytox Blue. Data represent n = 3 to 5 mice per group; shown as mean ± standard error of the mean. (B-C) WT and CGD mice were injected with PKH at 18 hours post–zymosan injection (post Zym), and tissues were collected 24 hours later (42 hours post Zym). (B) Number of PKH⁺MoMacs recovered from each of the indicated tissues. Bars show mean ± standard error with n = 3 to 5 mice per group representative of 2 independent experiments (*P < .05). (C) Sections of omenta from WT (left) or CGD (right) were stained as indicated. PKH⁺ F4/80⁺ MoMacs from the peritoneal cavity are denoted by green arrows; original magnification, 10×. Images were acquired by using a Marianas fluorescence microscope and analyzed by using SlideBook software (3i). DAPI, 4′,6-diamidino-2-phenylindole; DIC, differential interference contrast; N.D., not detected.