The diaphragm is a site of MoMac accumulation in CGD mice. (A) Hematoxylin and eosin–stained sections of diaphragm collected at 72 hours post–zymosan injection (post Zym) from WT (n = 3 mice shown, labeled i-iii) and CGD (n = 2 mice shown, labeled iv-v). Images captured at 4× magnification (top) or 20× (below). SM denotes diaphragm smooth muscle, PC denotes the peritoneal cavity. (B) Immunofluorescence staining of F4/80 with Ly6G (left) or Fibrin(ogen) (right) of diaphragm tissues from CGD mice at 72 hours after zymosan injection. Images were captured at 40× magnification, and orientation of the tissue with respect to the peritoneal cavity is indicated by the arrow below. (C) Quantitation of neutrophils (left) and MoMacs (right) from digests of diaphragm-associated tissues over time after zymosan injection. Graphs show mean ± standard error with n = 3 to 6 mice per group representative of 2 independent experiments. (D) Phenotype of MoMacs recovered from digests of diaphragm tissue over time post Zym. (E) Immunofluorescence analysis of diaphragm tissues from WT and CGD mice at 42 hours post Zym from animals also treated with i.p. PKH at 18 hours post Zym. Original magnification, 10×. Green arrows indicate PKH-labeled MoMacs. *P < .05. DAPI, 4′,6-diamidino-2-phenylindole; DIC, differential interference contrast.