CD45-ADC conditioning enables efficient multilineage donor engraftment in congenic mice. WT B6 (CD45.2) recipients (n = 5 mice/group, 10- to 12-week-old female) were conditioned with isotype-ADC (3 mg/kg) or CD45-ADC (0.3 mg/kg, 1 mg/kg, or 3 mg/kg) on d-2, or 500 cGy (5 Gy) TBI on d-1, and transplanted with 20 × 10⁶ B6 (CD45.1) donor BM on d0. (A) Overall donor (CD45.1) engraftment in the PB of transplanted mice was assessed by flow cytometry. Each individual line represents a single mouse tracked over 16 weeks, as indicated. (B) Donor-derived (CD45.1) lineage subsets in the PB of transplanted mice were assessed by flow cytometry. (C) Immune cell subsets in BM were analyzed at 16 weeks post-BMT in transplanted recipients. (D) Frequencies of donor-derived (CD45.1) hematopoietic progenitor cells (LSKs) and HSCs in the BM of transplanted recipients were analyzed at 16 weeks post-BMT by flow cytometry. *P < .05 vs isotype-ADC (3 mg/kg), using 1-way ANOVA with posthoc Dunnett’s multiple comparisons test. Data in (C) and (D) represent mean ± SEM. Experiments were performed twice, and data shown are from 1 representative experiment.