Single-agent CD45-ADC results in complete donor chimerism in a minor mismatch transplant model. DBA/2 (H-2^d, CD45.2) recipients (n = 5 mice/group, 20- to 22-week-old females) were conditioned with 3 mg/kg isotype-ADC or CD45-ADC on d-3, or 900 cGy (9 Gy) TBI on d-1, and transplanted with 20 × 10⁶ CByJ.SJL(B6)-Ptprca/J (H-2^d, CD45.1) donor BM on d0. Two mice from the CD45-ADC group died 3 weeks posttransplant, and 1 mouse from the isotype-ADC group died at 14 weeks due to unknown causes. (A) Engraftment of donor cells (CD45.1⁺): overall chimerism and chimerism in lineage subsets in the PB of transplanted mice were assessed by flow cytometry. Each individual line represents a single mouse tracked over 20 weeks posttransplant as indicated. (B) Donor (CD45.1⁺)-derived hematopoietic progenitor cells (LSKs) and HSCs in BM were analyzed at 20 weeks posttransplant by flow cytometry. Data represent mean ± SEM. (A,B) Experiments were performed twice, and data shown are from 1 representative experiment. (B) *P < .05 vs 9 Gy TBI-treated mice using 1-way ANOVA with posthoc Dunnett’s multiple comparisons test.