Inhibition of APC enhances the response to laser-induced injury in mouse cremaster arterioles. (A) Time lapse images from representative vehicle-treated (top row) and APC monoclonal Ab1609–treated mice (bottom row) after laser injury in cremaster arterioles. Images are binary representations of 2D confocal fluorescence images overlaid on the brightfield. Platelets are shown in red, P-selectin⁺ area in green, and fibrin area in blue (overlay colors are shown in the color wheel). White arrow indicates the direction of blood flow. Scale bar in each image, 10 μm. (B-D) Graphs show CD41⁺, P-selectin⁺, and fibrin⁺ areas over time after laser injury of cremaster arterioles in vehicle-treated (red) and Ab1609-treated mice (blue). Values are mean ± standard error of the mean (SEM). (E-G) Graphs show area under the curve (AUC; mean ± SEM) for platelets and P-selectin and fibrin areas over time. Statistics were calculated using the Mann-Whitney test; n = 13 injuries from 3 vehicle-treated mice and 10 injuries from 3 Ab1609-treated mice.