No effect of FV Leiden mutation on hemostatic plug formation after jugular vein puncture injury in mice. (A-C) Graphs show platelet accumulation (A), P-selectin expression (B), and fibrin accumulation (C) on the intra- and extravascular sides of the mouse jugular vein after puncture injury. Red columns are WT and blue FV^Q/Q. Values are mean ± standard error of the mean. Statistics were performed using a 2-way analysis of variance with Bonferroni post hoc test for multiple comparisons; n = 8 WT and 6 FV^Q/Q mice. (D) Bleeding time after jugular vein puncture injury in WT and FV^Q/Q mice. Horizontal line is median and error bars interquartile range. Statistics were performed using the Mann-Whitney test. a.u., arbitrary unit; NS, not significant.