American Society of Hematology

Figure 2.

$PBMCs from severe COVID-19 patients form ASC speck at similar frequency to neutrophils. PBMCs were stained with DAPI (blue), an anti-CD66b antibody (neutrophil marker, green) and (A) an anti-ASC antibody (red), or (C) with an anti-H3cit antibody (red). (A) Representative images from widefield microscopy of PBMCs. Scale bar, 10 μm. (B) Quantification of CD66b+ cells (neutrophils) in PBMC fraction. (C) Representative images from widefield microscopy of PBMCs. Scale bar, 10 μm. (D) Quantification of CD66b− cells presenting a speck. (E) Quantification of CD66b+ cells (neutrophils) with nucleus positive for H3cit. Each dot represents 1 individual. Values are means plus or minus SEM. Data were analyzed by Mann-Whitney U test; *P < .05. SEM, standard error of the mean.$

PBMCs from severe COVID-19 patients form ASC speck at similar frequency to neutrophils. PBMCs were stained with DAPI (blue), an anti-CD66b antibody (neutrophil marker, green) and (A) an anti-ASC antibody (red), or (C) with an anti-H3cit antibody (red). (A) Representative images from widefield microscopy of PBMCs. Scale bar, 10 μm. (B) Quantification of CD66b⁺ cells (neutrophils) in PBMC fraction. (C) Representative images from widefield microscopy of PBMCs. Scale bar, 10 μm. (D) Quantification of CD66b⁻ cells presenting a speck. (E) Quantification of CD66b⁺ cells (neutrophils) with nucleus positive for H3cit. Each dot represents 1 individual. Values are means plus or minus SEM. Data were analyzed by Mann-Whitney U test; *P < .05. SEM, standard error of the mean.

This Feature Is Available To Subscribers Only