Human neutrophils activate inflammasome early after LPS activation. Neutrophils isolated from healthy individuals (n = 5) were activated for different lengths of time with 5 μg/mL of LPS and stained with DAPI (blue) and an anti-ASC antibody (red). (A) Representative images from widefield microscopy of neutrophils activated for 30 minutes with 5 μg/mL of LPS. White arrows indicate the presence of the ASC speck. Scale bar, 10 μm. (B) Quantification of neutrophils presenting a speck at 30 minutes, 1 hour, and 4 hours after LPS activation. (C) IL-1β concentration measured in the supernatant of unstimulated (US) neutrophils or activated with LPS. Each dot represents 1 individual. Values are means plus or minus SEM. Data were analyzed by Mann-Whitney U test; *P < .05. SEM, standard error of the mean