Asymmetric inheritance of lysosomes and cell fate markers in human CD49fHSCs divisions. (A) Experimental design. (B) Representative video frames of dividing CD49fHSCs (quantification displayed in panel C) with symmetric and asymmetric LysoBrite inheritance. Scale bar: 10 μm. (C) Representative LysoBrite fluorescence intensity quantification over time of CD49fHSC daughter cells for a-/symmetric inheritance. Fold sister differences early (up to 60 minutes) after division are indicated, respectively. (D) Quantification of sister ratios directly after division. LysoBrite sister cell ratios are higher than for CD33, CD201, CD71, TMRM, CD49c, and CD34. LysoBrite sister cell ratios >3× and <1.5× were considered as a-/symmetric, respectively. n = 5 independent experiments for LysoBrite, CD33, CD201, CD71, and CD49c. n = 4 for CD34 and TMRM with 426, 260, 260, 166. 166, 207, and 211 CD49fHSC divisions analyzed for LysoBrite, TMRM, CD71, CD33, CD201, CD49c, and CD34, respectively.