DNA replication, cell-cycle, and DNA damage response are among the top negatively selected pathways in screen. (A) The number of genes negatively selected at days 4, 11, and 18 in Srsf2^P95H/+ (pink) and Srsf2^+/+ (blue) cells. The top 5 negatively selected genes for Srsf2^P95H/+ cells ranked by β score of all 3 cell lines at each timepoint are listed. FDR < 0.01, β score <−1, and any CEG2 essential genes are excluded. (B) Pathway analysis of the negatively selected genes for Srsf2^+/+ and Srsf2^P95H/+ at 3 timepoints showing DNA replication, cell-cycle, and DNA damage response pathways have a more negative effect on Srsf2^P95H/+ cell survival when perturbed.