Neutrophil ADCC of B-cell lymphoma cells is dependent on CD11b/CD18 integrins and is independent of ROS and proteases. (A) Neutrophil ADCC of Raji wild type or CD47KO cells opsonized with rituximab in the absence or presence of SSG . Neutrophil CD18 integrin was blocked using anti-CD18 f(ab′)2 fragments (n = 4-6 donors from 3 independent experiments). (B) ADCC toward Raji wild type or CD47KO cells opsonized with rituximab in the absence or presence of SSG by neutrophils from 4 healthy donors (left) or from 3 patients with chronic granulomatous disease (CGD; right), which are unable to produce ROS. Note that ADCC is comparable between these 2 groups. Data shown were obtained from 3 independent experiments. (C) Neutrophil ADCC of Raji wild type or CD47KO cells opsonized with rituximab in the absence or presence of SSG. Neutrophils were treated with DFP which reduces proteolytic activity (n = 6 donors from 3 independent experiments). Data are mean ± SEM. *P ≤ .05; ***P ≤ .001; ****P ≤ .0001.