JAM-C signal co-localizes largely with BVs and CD138. Representative LSFM of intact long bones of an MM-bearing mouse. (A) Channel signals for BVs are shown in red (CD144-AF647) and CD138 signals are shown in green (CD138-AF488). (B) Channel signals for BVs are shown in red and JAM-C signals are shown in blue (AF555). (C) Overlay of panels A and B. Arrows indicate MM cells in proximity with BVs display remarkably high JAM-C signal and low CD138 expression. (D) Graphical analysis of co-localizing MM cells with JAM-C-signal within the BM of MM-harboring mice (n = 5). Error bars indicate standard deviation (SD).