Figure 6.
Triple combination downregulates c-MYC while upregulating GILZ in vivo. (A) Principal component analysis of gene expression between the vehicle (blue) and triple combination (red). ATL43Tβ (-) tumor isolated from NSG mice on day 5 after treatment with I-BET762 (10 mg/kg), copanlisib (5 mg/kg), and bardoxolone methyl (2.5 mg/kg) (n = 4 per group). (B) Volcano plot showing fold changes (fold change >1.5 and P < .05) of differentially expressed gene-set between triple combination vs vehicle. (C) Quantitative real-time PCR analysis of TSCD22D3 (GILZ), PIK3IP1, TP53I3, MYC, TNFRSF8 (CD30), CDK6, and BATF3 transcripts (n = 3 to 4). (D) Hallmark gene set enrichment analysis pathways in ATL43Tβ (-) tumor isolated from NSG mice. (E) Tumor-associated genes depicted on heatmap analysis. Unpaired Student t test was used for statistical analysis. *P < .05, **P < .01, ****P < .0001.

Triple combination downregulates c-MYC while upregulating GILZ in vivo. (A) Principal component analysis of gene expression between the vehicle (blue) and triple combination (red). ATL43Tβ (-) tumor isolated from NSG mice on day 5 after treatment with I-BET762 (10 mg/kg), copanlisib (5 mg/kg), and bardoxolone methyl (2.5 mg/kg) (n = 4 per group). (B) Volcano plot showing fold changes (fold change >1.5 and P < .05) of differentially expressed gene-set between triple combination vs vehicle. (C) Quantitative real-time PCR analysis of TSCD22D3 (GILZ), PIK3IP1, TP53I3, MYC, TNFRSF8 (CD30), CDK6, and BATF3 transcripts (n = 3 to 4). (D) Hallmark gene set enrichment analysis pathways in ATL43Tβ (-) tumor isolated from NSG mice. (E) Tumor-associated genes depicted on heatmap analysis. Unpaired Student t test was used for statistical analysis. *P < .05, **P < .01, ****P < .0001.

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