SPFQ-ABL1 and BCR-ABL1 differentially phosphorylate SHIP1 and GAB2. (A) Volcano plot of peptides identified in DPU analysis. Plots show log fold change (logFC) on the x-axis and $-$log10(P value) on the y-axis. Proteins/peptides of interest are labeled with gene name. Significantly enriched (P = .05) peptides and peptide modifications are colored according to the key in the top left of the plot. (B) Protein schematics of SHIP1 and GAB2 showing differential phosphorylation events identified in DPU analysis, produced by using ProteinPaint (Zhou et al Nature Genetics. 2016). Tyrosine phosphorylation events and resultant effects are coded according to the key in the top left corner. Functional domains are colored according to the key below each protein. (C) western blot analysis of SHIP1 and Phospho-AKT (P-AKT) expression in Ba/F3 cells expressing MSCV, BCR-ABL1, or SFPQ-ABL1. Pu.1/Irf4 DKO (representative western blot image is shown, n = 4). (D) western blot analysis of Ba/F3 cells treated with a dose titration (100 nM, 1 µM, and 10 µM) of imatinib for 6 hours (representative western blot image is shown, n = 3). (E) western blot analysis of SHIP1 and P-AKT in Pu.1/Irf4 DKO cells expressing MSCV, BCR-ABL1, or SFPQ-ABL1. Cell lines #1 to #3 represent 3 biologically independent cell lines.