Comparison of GPR34 and its various mutants in activation of various signaling pathways using reporter assays. This was performed in isogenic Flp-InTRex293 cell lines that stably express a single copy of GPR34 or its mutant. Data (mean ± standard deviation) presented are from 3 independent experiments. In each reporter assay, the data are normalized to the reference control without LysoPS stimulation. Comparisons between various groups were assessed with one‐way analysis of variance with significant differences indicated (*P < .05; **P < .01; ***P < .001; ****P < .0001). Control: the parental Flp-In T-REx-293 cell line. Wild-type (WT) or various mutations indicated: the derived stable expression cell line with single copy of WT or mutant GPR34.