The relationship between the GC-microenvironmental signature and multiomics prognostic factors. (A) Integrated visualization of transcriptome data from nCounter analysis, genetic alterations, and COO classification combined with DMS score in 170 DLBCL cases. DMS scores were calculated based on enrichment of ICOS (follicular T cells), CD11c (DCs/macrophages), and FGFR1 (stromal cells). Gene expression levels were shown as percentile of the normalized expression values in each gene. CNA burden was represented by the number of genes involved in the CNA region. Tumor activity-related signatures, such as expression levels of proliferation-related genes or the number of genes showing CNA and BCR-TLR signaling mutations, were enriched as the DMS score decreased. (B) The difference of MYC protein expression by DMS score. MYC protein positivity was analyzed by IHC. P values were calculated using the Steel-Dwass test. (C) MYC and BCL2 protein levels were assessed by immunohistochemistry, and cases positive for both MYC and BCL2 were defined as DEL. DELs (magenta) exhibited low DMS scores. Fisher’s exact test was performed to calculate P value. (D) The frequency of DHL by each DMS score is shown. Fisher’s exact test was performed to calculate P value.