Myeloid cell–specific PKM2^−/− mice exhibited improved local cerebral blood flow and reduced poststroke cerebral thrombosis. (A) Left: representative images were taken by using laser speckle imaging of regional cerebral blood flow in the cortical region. Right: quantification at different time points (5 to 120 minutes). (B) Left: representative immunostaining images for platelets (CD41⁺, green; fibrinogen, red). Scale bar, 100 μm. Right: thrombotic index as defined by the ratio of occluded brain vessels to the total brain vessels in the ipsilateral hemisphere. (C) Brain homogenates from the infarcted and peri-infarcted area after 1 hour of ischemia and 23 hours of reperfusion were processed for western blotting: representative western blots and densitometric analysis of fibrinogen and platelets (CD4⁺). β-actin was used as a loading control. All data are from male mice and are mean ± SEM; n = 5 (A); n = 4 (B-C). Data are from 2-way repeated measures ANOVA (Kruskal-Wallis test) followed by Fisher’s LSD test (A), or an unpaired Student t test (B-C). I/R, ischemia and reperfusion.