ML265-treated male WT mice exhibited significantly reduced infarct area and improved long-term sensorimotor outcomes. (A) Schematic of experimental design. (B) Left: representative magnetic resonance imaging from one mouse of each group on day 1 in filament model. White is the infarct area. Right: corrected mean infarct area of each genotype. (C) mNSS in the same mice at weeks 1, 2, 3, and 4 based on spontaneous activity, symmetry in the movement of 4 limbs, forepaw outstretching, climbing, body proprioception, and responses to vibrissae touch (higher score indicates a better outcome). Sensorimotor recovery in the same mice as analyzed by right turn ratio in corner test (D), fall latency in accelerated rota-rod test (E), and motor strength in hanging-wire test (F). The data in panels C-F are in box plots and the horizontal bars indicate median value. (G) Survival rate between day 0 and day 28. Data are mean ± SEM (B) and median ± range (C,F); n = 15-16 mice. Data are from an unpaired Student t test (B), or 2-way repeated measures ANOVA (Kruskal-Wallis test) followed by Fisher’s LSD test (C,F). Comparison of survival curves was evaluated by log-rank (Mantel-Cox) test (G).