SIRT1 expression modulated mitochondrial activity. (A) Heatmap showing the differentially expressed genes in Nalm6 CON, sgSIRT1(sg), and SIRT1 OE cells (log fold change >1 or <−1, adjusted P < .05). (B) KEGG pathway enrichment analysis of the differentially expressed genes in Nalm6 CON and SIRT1 OE cells. Top 15 enriched pathways with adjusted P < .05 are shown in the bubble plot. (C-D) GSEA analysis of the oxidative phosphorylation gene expression signature for SIRT1 OE vs Nalm6 CON (C) and sgSIRT1 vs Nalm6 CON (D). (E) Membrane potential in Nalm6 CON and sgSIRT1 and SIRT1 OE cells were evaluated by TMRM staining. Data represent mean ± SD (n = 3). Data with statistical significance are as indicated: *P < .05, ***P < .005.