Contribution of EV secretion and cell adhesion–related genes to MM patient prognosis. (A) A scatter plot showing the relationship between LAMP2 and SORT1 expression in the 6 cell lines used in this study. The x-axis indicates LAMP2 expression, and the y-axis indicates SORT1 expression. (B) Scatter plot showing the relationship of LAMP2 and SORT1 expression in a publicly available Cancer Cell Line Encyclopedia data set. Each dot indicates an MM cell line; red, cell lines in our data set; blue, cell lines that are known to be partially resistant or resistant to lenalidomide. (C) Uniform manifold approximation and projection (UMAP) plot classifying patient outcomes as aggressive or passive. (D) UMAP plot of MM cells focusing on the PC1 signature (left). Violin plot of PC1 signature genes among patient states (right). (E) UMAP plot of MM cells focusing on the PC2 signature (left). Violin plot for PC2 signature genes among patient states (right). (F) Violin plots for LAMP2 and SORT1 gene expression among patient states. (G-H) Violin plots for EV biogenesis-associated gene expression among patient states, among PC2 signature genes (G) and typical EV marker (H).