Impact of the MV injection on the mouse hemostatic balance. (A) Cecal ligation was performed on CD-1 mice on 3 kinetic, D1: 24 hours after sepsis induction; D2: 48 hours after sepsis induction; D5: 5 days after sepsis induction; n = 36 per group. Each group of animals received a daily IV tail injection of uPA-Gran-MVs (red), Gran-MVs (blue), or SPN uPA-Gran-MVs (green) from D1 (24 hours after surgery) and compared to a group of healthy mice (control) (purple). Once sepsis was established, the mice were observed daily to assess spontaneous mortality and monitored to measure their heart rate, oxygen saturation, breathing rate, and body temperature. In according to the kinetic, the animals were sacrificed at D1, D2, or D5. (B-F) Measure of fibrinolytic and procoagulant parameters at D1, D2, and D5: plasmin-anti-plasmin complex (PAP), MV dependent plasmin generation capacity (MV-PGC), thrombin-anti-thrombin complex (TAT), MV dependent tissue factor activity (MV-TF), and d-dimer, respectively. Data are mean ± SEM. *P < .05; **P < .01; NS: not significant.