ANGPTL2 regulates G0s2 expression through Ppard. (A) Relative Ppard mRNA expression in LT-HSCs from Angptl2^fl/fl and Cdh5-Cre; Angptl2^fl/fl mice as measured by quantitative RT-PCR (n = 3). (B) Relative Ppard mRNA expression in LT-HSCs from Angptl2^fl/fl and Tie2-Cre; Angptl2^fl/fl mice as measured by quantitative RT-PCR (n = 3). (C) Relative luciferase activities were determined in 32D cells cotransfected with the Ppard plasmid and luciferase reporter containing the G0s2 promoter (n = 3). (D) Chromatin immunoprecipitation (ChIP) assays were performed in Lin⁻ BM cells. The G0s2 promoter regions bound to endogenous PPARD were measured by PCR. (E) The G0s2 mRNA expression levels in Ppard-overexpressing (Ppard-OE) Lin⁻ BM cells were detected by qRT-PCR (n = 3). (F) The G0s2 mRNA expression levels were measured in LT-HSCs upon the treatment with PPARD agonist (GW501516) for 24 hours by qRT-PCR (n = 3). (G) Schematic diagram of the working model for the role of endothelial cell-derived ANGPTL2 in HSC stemness. *P < .05; ***P < .001.