Effect of overexpression of LCP1 I232F in 32D cell proliferation. (A) 32D cells expressing LCP1 I232F display impaired cell proliferation with unchanged viability upon proliferation with murine IL-3–containing media (2 ng/mL). (B) Images, obtained at ×40 magnification using a Leica DM6B upright microscope, show that LCP1 I232F-expressing cells have granulocytic dysplasia with the appearance of large multinucleated cells. (C) The cell cycle analysis for 32D cells harvested at indicated time points using DNA staining with propidium iodide followed by flow cytometric analysis (left) shows arrest of cell cycle at G2M Phase in LCP1 I232F-expressing cells (right). Data are shown as mean ± standard error of mean (results of n = 3 biological replicates unless otherwise specified). Statistical analysis was assessed by Student t test. *P < .05, **P < .01, ***P < .001, ****P < .0001. ns, not significant.