Delayed administration of caADAMTS13 restores CBF, reduces infarct volume, and attenuates IgG leakage at 6 hours post-I/R injury in mice. (A) Representative LCSI raw data with defined ipsilateral and contralateral ROIs. (B) Ratios of ipsilateral and contralateral flux values (mean ± standard deviation, n = 8-10) are shown for each time point confirming transient occlusion of the MCA at time = 0 as indicated by flux ratios <0.5 in both treatment groups. (C) Flux ratios were normalized to that of the baseline measurement (dotted line). Error bars represent mean ± standard deviation. Divergence of flux ratios between the 2 treatment groups at each imaging time point was assessed using 2-way ANOVA followed by Sidak’s multiple comparisons test. *P < .05, **P < .01. (D-E) Infarct volume and IgG staining volume, respectively, were determined from whole-brain imaging performed using Slidescanner. Shown are representative images, at an approximately equivalent coronal level, for 2 animals in both treatment groups. Single comparisons were performed using an unpaired Student t test. *P < .05.