Clonal architecture of TP53-mutated MPN patients. Phylogenetic trees show the order of mutation acquisition during MPN history with maximum likelihood in 8 patients. The mutational history was manually reconstructed from the single-cell data. In the case of MPN3, it was not possible to infer the order of mutation acquisition in JAK2 and U2AF1 because of the absence of cells with wild-type version of these genes. Each circle represents a clone identified in single-cell analysis using Tapestri platform and Tapestry Insights software (Mission Bio). The size of each circle denotes the relative clone size. The nomenclature of each mutation is indicated only when 2 or more mutations were present in the same gene.