Disulfide connectivity and structural architecture of the VWF C8-3 subunit. (A) Disulfide connectivity of the C8-3 subdomain of the VWF D′D3 assembly. (B) Location of p.(Cys1084) in the crystal structure of the VWF C8-3 subdomain. Intra-subdomain disulfide bonds are annotated in green, the p.(Cys1060)-p.(Cys1084) disulfide bond in yellow, and the cysteine involved in inter-subunit bonding p.(Cys1099) in red. Protein Data Bank 6N29, accessed 20 March 2021. Image rendering was performed with PyMOL software (version 2.1.1).