MK maturation and PPF were expedited in LdhA^−/− mice. (A) Representative Annexin V⁺ gating strategy and percentage of apoptotic platelets in LdhA^f/f and LdhA^−/− mice (n = 6). (B) Biotin-labeled ratio of platelets at different time points after tail IV injection of Sulfo-NHS-LC-Biotin (30 μg/g weight) in LdhA^f/f and LdhA^−/− mice (n = 5; 8 weeks old, male). (C) Representative thiazole orange–positive gating strategy and percentage of reticulated platelets in LdhA^f/f and LdhA^−/− mice (n = 5). (D) Platelets were eliminated by tail IV injection of anti-CD42b antibody (2 μg/g weight) in LdhA^f/f and LdhA^−/− mice, and platelet counts were monitored at different time points (n = 5; 8 weeks old, male). (E) Detection of plasma TPO level by using enzyme-linked immunosorbent assay (n = 12). (F) Quantitative analysis of bone marrow MKs in LdhA^f/f and LdhA^−/− mice femur bone by immunohistochemistry with 5 μg/mL anti-CD42c antibody (n = 9; 8 weeks old, male). Scale bar, 50 μm. (G) Representative CD41⁺CD42b⁺ MK gating strategy. (H) Statistical analysis on the ratios of CD41⁺CD42b⁺ MKs in LdhA^f/f and LdhA^−/− mice bone marrow, spleen, and lung according to flow cytometry (n = 3; 8 weeks old, male). (I) Immunofluorescence images of PPF-MKs derived from the fetal liver in LdhA^f/f and LdhA^−/− pregnant mice; statistical analysis on PPF-MK/total MK ratio is shown (n = 3). Scale bar, 30 μm. (J) Detection of polyploidy (2N, 4N, and 8N) from fetal liver MKs of LdhA^f/f and LdhA^−/− pregnant mice by flow cytometry (n = 4). *P < .05, **P < .01, ***P < .001, ****P < .0001. DAPI, 4′,6-diamidino-2-phenylindole; ns, not significant; SSC-A, side scatter area.