Stored platelet (PLT) posttransfusion function in mice. (A) Outline of the mouse PLT transfusion model (details provided in “Methods”). (B) PLT in vivo survival after storage for 24 hours at either RT (red circles) or 4°C (blue squares; n = 9). (C) αIIbβ3 integrin activation in whole blood 4 and 24 hours after PLT transfusion with either 24-hour RT- (red circles) or 4°C-stored (blue squares) PLTs after stimulation with 100 nM of convulxin (n = 9; shown as mean fluorescence intensity [MFI] ± standard error of the mean [SEM] of Jon/A antibody binding). (D) GPVI expression 4 and 24 hours after transfusion of either 24-hour RT- (red circles) or 4°C-stored (blue squares) PLTs (n = 9; shown as MFI ± SEM of JAQ-1 with goat anti-rat immunoglobulin G–phycoerythrin secondary antibody). *P = .032 (4 hours) (C) and P = .038 (4 hours) (D), **P = .001 (4 hours: RT vs 4°C) (B), P = .012 (24 hours) (C), and P = .008 (24 hours) (D). CVX, convulxin; RBC, red blood cell; WT, wild type.