DMT1 protein expression is strongly induced in male mice by dietary iron deprivation for 14 days. Eight-week-old mice were fed a control diet (50 ppm iron) or a low-iron diet (2-6 ppm iron) for 0.5, 1, 2, 4, 8, 10, or 14 days (n = 5 for each diet at each time point). Upon sacrifice, BBMVs were isolated from duodenal scrapes, liver was collected for nonheme iron analysis, and blood was collected for Hb measurement. Liver nonheme iron content was lower after 14 days of dietary iron deprivation (A) (*P < .05; Student’s t test), but blood Hb levels were unaffected (B) (Student’s t test). Western blot analysis of proteins from duodenal BBMV preps was also undertaken. Quantification of data from multiple experiments showed that DMT1 was most strongly and reproducibly induced by 14 days of low-iron feeding (C) (n = 3-6 per group). Data were analyzed by Kruskal-Wallis 1-way ANOVA followed by Dunn’s multiple comparison test (**P < .01). Also shown are representative time course DMT1 western blots of duodenal proteins isolated from control and iron-deprived mice (D-F). Each lane of the western blot represents 1 mouse. β-actin was used as a loading control (shown below each DMT1 blot).