Integrative gene expression, alternative splicing, and transcript usage analyses categorize gene targets of mutant SF3B1. (A) PCA plots based on gene expression of the 3000 most highly variable genes color-coded by SF3B1 mutation status and sign-coded by patient ID. (B) Heatmap showing distance of the indicated iPSC-HSPCs based on pairwise Pearson correlation of their gene expression profiles, color-coded by SF3B1 mutation status and patient ID. (C) PCA plot based on inclusion levels of the differentially spliced events between SF3B1^K700E and SF3B1^WT iPSC-HSPCs. (D) Scatterplots comparing the mean inclusion levels of the differentially spliced events in SF3B1^K700E vs SF3B1^WT iPSC-HSPCs with different event types broken down by color, as indicated. (E) Schematic summarizing the integrative analysis used to derive a mutant SF3B1 signature of splicing events and genes and scatterplot showing inclusion level difference of all 59 signature splicing events, corresponding to 34 genes. A positive y axis value indicates that the event is more frequently found in SF3B1^K700E vs SF3B1^WT cells.