Events of the mutant SF3B1 splicing signature. Heatmap showing the row normalized inclusion levels of the 59 signature events across HSPCs from all iPSC lines. For each row, color-coded side panels present metadata relevant to each event, including the log₂fc of expression of the respective genes, the biotypes of the up- and downregulated transcripts that are associated with the splicing events, and the presence of the events in the MDS patient dataset of Pellagatti et al,⁹  encoded as not present (signature events not present in any comparison); present but not significant (signature events that were not statistically significant or/and had an absolute inclusion level difference < 0.1 in both comparisons [SF3B1mut vs SF-WT and SF3B1mut vs WT, ie, healthy individuals]); present only in SF3B1mut vs SF-WT (signature events statistically significant [FDR < 0.05] and with an absolute inclusion level difference > 0.1 only in the SF3B1mut vs SF-WT MDS patient comparison); and present in SF3B1mut vs SF-WT and SF3B1mut vs WT (signature events statistically significant [FDR < 0.05] and with an absolute inclusion level difference > 0.1 in both comparisons). The annotations of the transcript biotypes are derived from the Ensembl GRCh37 gtf annotation file. Each row represents one event labeled with the respective gene name followed by a number indicating distinct events.