The GPVI collagen-binding interface. (A) Surface representation of the GPVI collagen-binding site (magenta) with residues situated within 4.5 Å of collagen peptide (GPO)₅ shown in sticks representation and β-strand labeled in green. (B) Representation of conservation within the GPVI family using ConSurf⁵² coloring illustrates strict conservation of six central and more variability in five surrounding residues in the collagen-binding site. (C) The GPVI-(GPO)₅ interface showing hydrogen bonding interactions and salt bridges (dashed lines) and intermolecular carbon–carbon distances within 4.5 Å (thin solid lines). (GPO)₅ residues in the leading (pink), middle (magenta), and trailing (orange) chain are shown in sticks representations and labeled by a sequence number (gray). Also displayed is the (GPO)₅ surface, highlighting regions within 4.5 Å of GPVI by coloring according to the nearest chain. GPVI residues in the interface are shown as main chain ribbons and sidechain sticks (blue) and labeled by residue (black). (D-E) Detailed views of the hydrogen bonding interactions between (GPO)₅ and GPVI residues Gln71 and Trp76 (D) and Arg38, Glu40, and Arg67 (E). Hydrogen bonds and salt bridges are indicated by dashed lines.