Mutational analysis of GPVI collagen binding. Binding of GPVI mutants (colored series) to (A) CRP, (B) fibrillar collagen I, and (C) Toolkit-peptide III-30 as measured in solid-state binding assays. Data measured as A₄₅₀ is normalized to the binding of WT GPVI (black series). All data points represent the mean ± SD of at least 3 independent experiments. Binding curves are fitted to the equation Abs = (B_max × c)/(K_D,app + c) using nonlinear regression in SigmaPlot, where c is the GPVI concentration (μM). See also supplemental Figure 6.