Unsupervised hierarchical clustering of DNA methylation profiles and associations between DNA methylation clusters and additional parameters. (A) Heatmap of the DNA methylation profiles of 64 AMLs based on unsupervised hierarchical clustering. Clustering was based on the 567 CpG sites with the most variable methylation values in the 64 studied cases. Four clusters were generated: 1, 2, 3, and 4. DNA methylation levels were classified into 3 groups according to their β value: hypermethylation (≥0.67), intermediate methylation (0.34-0.66), and hypomethylation (≤0.33), respectively. Light blue, orange, and dark orange indicate the presence of the specified mutation, high gene expression, and chromosomal aberration, respectively. Brown indicates KMT2A-MLLT3 fusion, and dark blue indicates FLT3-ITD with high allele ratio (>0.7). Purple and black indicate non–complete remission (CR) and events and deaths, respectively. (B) Comparison of the Kaplan-Meier curves of OS between clusters 1 and 2. KMT2A-R, KMT2A rearrangement; PTD, partial tandem duplication.